pTDP-43 Quantification Summary (for UCLA Request)

	A	B	C	D	E	F	G	H	I
1	Notes: All slides were stained with TIP-PTD-M01A antibody at 1:10,000 dilution unless stated otherwise
2	Case #	Diagnosis	Section	Consensus	Comments/Notes	Ratings AM	Ratings KO	Diff ratings	Stain Initial/Date

3	62	sALS	RG1L	mild	used as example image in slides	moderate signal, predominantly glia	moderate pTDP signal		AM240119
4	62	sALS	RG2L	weak		weak signal	little to no pTDP signal		KO231129
5	63	sALS	RG1L	mild		weak-moderate signal, predominantly cytoplasmic	mild pTDP signal		AM240119
6	63	sALS	RG2L	weak		weak signal (total of 4 loci counted)	no pTDP signal		AM240119 & KO240126 (restain)
7	66	sALS	RG1F	moderate	used as example image in slides	moderate signal (both remount and restain)	little pTDP signal (remounted)		AM240119 & KO240126 (restain)
8	66	sALS	RG2L	frequent	used as example image in slides	strong signal	moderate pTDP signal		AM240119 & KO240126 (restain)
9	68	sALS	RG1T	mild		weak-moderate signal	mild to moderate pTDP signal		KO231129
10	68	sALS	RG2T	mild	used as example image in slides	moderate signal	mild pTDP signal		AM240119 & KO240126 (restain)
11	69	sALS	RG1L	frequent	used as example image in slides	strong signal	moderate pTDP signal		AM240119
12	69	sALS	RG2L	frequent		moderate signal	frequent pTDP signal		AM240119 & KO240126 (restain)
13	75	sALS	RG1A	mild		weak signal	mild pTDP signal		AM240119 & KO240126 (restain)
14	75	sALS	RG2L	moderate	Used as example image in slides	moderate signal, highly localized (left part of the section)	moderate pTDP signal		AM240119 & KO240126 (restain)
15	92	sALS	AFP	none		weak signal	no pTDP signal		AM240119 & KO240126 (restain)
16	92	sALS	MC LEG	moderate	used as example image in slides	moderate signal, highly localized (btm right of section)	moderate pTDP signal		KO231201
17	109	sALS	AFP	none		high BG, weak signal	little to no pTDP signal		AM240119
18	109	sALS	MC MED	none	used as example image in slides	no signal	no pTDP signal		KO231201
19	110	sALS	MID FRONTAL	none		no signal	little to no pTDP signal		KO231214
20	110	sALS	MC MED	mild		weak-moderate signal	mild to moderate pTDP signal		KO231129
21	111	sALS	AFP	none	used as example image in slides	no pTDP signal	no pTDP signal		AM240123
22	111	sALS	MC MED	weak		mild pTDP signal (<10)	mild pTDP signal		AM240123
23	112	sALS (?)	AFP	none		no pTDP signal	no pTDP signal		AM240123
24	112	sALS (?)	MC MED	weak		weak signal, predominantly glia/white matter	mild pTDP signal		AM240123
25	113	sALS (?)	AFP	weak		weak signal	mild pTDP signal		KO231201
26	113	sALS (?)	MC MED	frequent (see note)	pTDP signal appears to be most frequent throughout the darker purple band along the edge of the tissue section	mild-medium pTDP signal, strange band of signals in upper lamina (II?)	moderate to frequent pTDP signal		AM240123
27	116	sALS	AFP	none		no pTDP signal	no pTDP signal		AM240123
28	116	sALS	MC MED	mild		mild to moderate pTDP signal	mild to moderate pTDP signal		AM240123
29	121	sALS	AFP	frequent		moderate with laminal II concentration	frequent pTDP signal		AM240123
30	121	sALS	MC MED	frequent	used as example image in slides	strong signal	moderate to frequent pTDP signal		AM240123
31	125	sALS	AFP	none	used as example image in slides	no signal	no pTDP signal		AM240123
32	125	sALS	MC MED	none		no signal	no pTDP signal		AM240123
33	127	sALS	AFP	none		no signal	no pTDP signal		AM240123
34	127	sALS	MC MED	weak		weak signal	mild pTDP signal		AM240123
35	128	sALS	MID FRONTAL	weak		little to no pTDP signal	little to no pTDP signal		KO231214
36	128	sALS	MC MED	moderate		moderate to frequent pTDP signal	moderate to frequent pTDP signal		KO231214
37	130	sALS	AFP	none		no pTDP signal	no pTDP signal		KO231129
38	130	sALS	MC MED	none	used as example image in slides	no signal	no pTDP signal		AM240123
39	94	sALS	MC	frequent	Old MC slide from Extras box	frequent pTDP signal	frequent		KO240126
40	97	sALS	MC FACE	none	Old MC face slide from Extras box	no pTDP signal	none		KO240126
41	107	sALS	MC MID	weak	Has some Betz cells	mild pTDP signal	weak		KO231129
42	93	sALS/FTD	MID FRONTAL	frequent	Old image; Upon discussion of tissue stained on 072414, all orange signals were quantified and the consensus is frequent cytoplasmic pTDP signal	frequent. Weak staining overall, lots of NCI and GCI, inclusions are light and granular	frequent pTDP signal		072414
43	93	sALS/FTD	MC	frequent	Old image; Some degree of heterogeneity in tissue. Cells with NCI are concentrated in one region, weak neuronal signal elsewhere	frequent. Some degree of heterogeneity in tissue. Cells with NCI are concentrated in one region, sparse and weak neuronal signal elsewhere	moderate pTDP signal		072414
44	96	sALS/FTD	AFP	moderate	new slides made 240307 AM; mostly NCI throughout laminae	moderate	mild pTDP signal		KO240315
45	96	sALS/FTD	MC	mild	Old MC slide from Extras box	frequent. Concentrated in outer layers (III?) predominantly NCI and neuropil grains	moderate pTDP signal		KO231214
46	99	sALS	MID FRONTAL	frequent	Old image; Upon discussion of tissue stained on 112414, all orange signals were quantified and the consensus is frequent cytoplasmic pTDP signal	mild-moderate, mostly glial	moderate to frequent pTDP signal		112414
47	99	sALS	MC	moderate	Old image; grainy/speckled signal throughout the tissue and not just in or near cells but did not factor those into the quantification. Only quantified the stronger looking signals that were in or near cells.	moderate, lots of grains and DN, highBG	moderate pTDP signal (only counted the stronger looking signals)		not sure
48	108	sALS	AFP	none	new slides made 240307 AM	none	no pTDP signal		KO240315
49	108	sALS	MC	frequent	Old image	frequent, NCI, high BG	moderate pTDP signal		not sure
50	132	sALS	AFP	weak		weak	little to no pTDP signal		KO240126
51	132	sALS	MC MED	moderate		moderate, mostly GCI, some NCI	moderate to frequent pTDP signal		KO240126
52	135	sALS	AFP	none		none	No pTDP signal		KO240126
53	135	sALS	MC MED	weak	Presence of Betz cells	weak	little to no pTDP signal		KO240126
54	142	sALS	AFP	weak	Lots of lipofuscin/brown signal, unsure if any of the red signals are true pTDP signal or artifact	mild, mostly GCI, few NCI	little to no pTDP signal		KO231201
55	142	sALS	MC MED	mild		weak	moderate to frequent pTDP signal		KO231201
56	143	sALS	AFP	none	new slides made 240307 AM	none	no pTDP signal		KO240315
57	143	sALS	MC MED	weak	new slides made 240307 AM	weak	mild pTDP signal		KO240315
58					Rating System Key
59					Consensus rating grades	AM rating grades	KO rating grades
60					none	No signal	none
61					weak	Weak signal	little to none-mild
62					mild	Weak-moderate signal	moderate
63					moderate	moderate	moderate-frequent
64					frequent	Strong	frequent
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