Vivantis Technologies Sdn Bhd

Description
Viva 2-step RT-PCR kit is specially designed to provide reliable synthesis of full-length cDNA and convenient application of cDNA in PCR. M-MuLV Reverse Transcriptase synthesize complementary DNA strand initiating from a specific primer, oligo d(T) or random hexamer. The absence of RNase H enhances the synthesis of long cDNA as RNA strand does not degraded in DNA-RNA hybrid during first strand cDNA synthesis. With variety of kit options for standard PCR and long PCR, Viva 2-step RT-PCR kit provides flexibility in an easy use format.

Features

• Absence of RNase H activity allows high amount of full length cDNA synthesis with RNA templates up to 10kb.
• Wide selection of primers, oligo d(T) or random hexamer. Taq DNA Polymerase and MaxTaq DNA Polymerase for amplification for short and long DNA fragments.

Kit Component

Viva cDNA Synthesis Kit

• 10X M-MuLV RT Buffer, 250µl
• 10,000u M-MuLV Reverse Transcriptase
• 10mM dNTPs mix, 0.25ml
• RNase-free water 3ml
• 40µM Oligo d( T ), 100µl
• 50ng/µl Random hexamer, 100µl

Viva PCR Kit

• 500u Taq DNA Polymerase / 500u Chromo Taq DNA Polymerase / 500u MaxTaq DNA Polymerase / 500u Chromo MaxTaq DNA Polymerase
• 10X PCR Reaction Buffers
• 50mM MgCl₂
• 10mM dNTPs Mix, 0.25ml

Storage

• Store at -20°C

Ordering Information

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Manual

Viva 2-Step RT-PCR kit with M-MuLV RT / Taq DNA Polymerase

Viva 2-Step RT-PCR Kit with M-MuLV RT / Chromo Taq DNA Polymerase

Viva 2-Step RT-PCR Kit with M-MuLV RT / MaxTaq DNA Polymerase

Viva 2-Step RT-PCR Kit with M-MuLV RT / Chromo MaxTaq DNA Polymerase

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MSDS
Viva 2-step RT- PCR Kit

Publication
This Product Has Been Used In:

Nazaktabar, Ahmad et al. (2022). Molecular typing of group A bovine rotavirus of calves in the provinces of Tehran, Alborz and Qazvin. Iranian Journal of Veterinary Research. 10.22059/ijvm.2012.30221.

Abbas Zamanizadeh et al. (2021)A comparison of two probiotics Aspergillus oryzae and Saccharomyces cerevisiae on productive performance, egg quality, small intestinal morphology and gene expression in laying Japanese quail, 20(1):232-242.

Naderi et al. (2021) Evaluation of Flavonol Synthase (FLS) gene expression in Cressa cretica L. using plant growth regulators, International Journal of Advanced Biological and Biomedical Research, 9(1): 64-76.

Al-Jallad et al. (2020) Molecular Characterization of Isolated Infectious Bronchitis Viruses from Affected Vaccinated Broiler Flocks in Syria, BMC Veterinary Research, 16:449.

Maryam Fatehi-Agdam et al. (2020) Efficacy of metformin and chemotherapeutic agents on the inhibition of colony formation and Shh/Gli1 Pathway: Metformin/Docetaxel versus Metformin/5-Fluorouracil, Drug Research, 71:17-25.

Asghari, M., Abazari, M.F., Bokharaei, H., Aleagha, M.N., Poortahmasebi,V., Askari, H., Torabinejad, S, Ardalan, A., Negaresh,N., Ataei, A., Pazooki, P., Poorebrahim, M (2018). Key genes and regulatory networks involved in the initation, progression and invasion of colorectal cancer , Future Science (2018).

Heydari-Mehrabadi, A. et al. (2018) Analysis of Polymorphism and Expression Profile of ASIC1 and IL-6 Genes in Patients with Gastric Cancer, . Asian Pacific Journal of Cancer Prevention,19(12):3451-3455.

Payehghadr, S. et al. (2018) Detection of miR-21, MUC1mRNA and VEGF Protein Biomarkers Expression Changes in Oral Squamous Cell Carcinomas (OSCC) in Peripheral Blood, . Asian Pacific Journal of Cancer Biology, 3(2):59-64.

Atari-Hajipirloo, S., Nikanfar, S., Heydari, A., Kheradmand, F. (2017). Imatinib and its combination with 2,5-dimethyl-celecoxib induces apoptosis of human HT-29 colorectal cancer cells, Research in Pharmaceutical Sciences Journal, , Vol. 12, No. 1. (2017).

Bina, P., Kakhki, M.P., Sahraian, M.A., Behmanesh, M. (2017) The expression of lnc-IL-7R long non-coding RNA dramatically correlated with soluble and membrane-bound isoforms of IL-7Ra gene in multiple sclerosis patients

Jaroonwitchawan, T., Chaicharoenaudomrung, N., Namkaew, J., Noisa, P. (2017). Curcumin attenuates paraquat-induced cell death in human neuroblastoma cells through modulating oxidative stress and autophagy Neuroscience Letters. 636. Pp.40-47

Sheikhalizadeh, V., Hasani, A., Rezaee, M.A., Rahmati-yamchi, M., Hasani, A., Ghotaslou, R., Goli, H.R. (2017) Comprehensive study to investigate the role of various aminoglycoside resistance mechanisms in clinical isolates of Acinetobacter baumannii Journal of Infection and Chemotherapy. 23(2) pp74-79

Sheikholeslami, A., Nabiuni, M., Arefian, E. (2017). Suppressing the molecular signaling pathways involved in inflammation and cancer in breast cancer cell lines MDA-MB-231 and MCF-7 by miR-590, Sage Journals, Vol. 39, No. 4 (2017).

Avci, E., Arıkoğlu, H., Kaya, D.E. (2016) Investigation of juglone effects on metastasis and angiogenesis in pancreatic cancer cells. Gene.588. Pp..74-78.

Babaei-Balderlou, F., & Khazali, H. (2016) Effects of Ghrelin on Sexual Behavior and Luteinzing Hormone Beta-subunit Gene Expression in Male Rats Journal of Reproduction Infertility2(1)17(2), p.88-96.

Jaroonwitchawan, T., Muangchang, P., Noisa, P. (2016). Inhibition of FGF signaling accelerates neural crest cell differentiation of human pluripotent stem cells. Biochemical and Biophysical Research Communications. 481(1-2). Pp.176-181

Majid Rahmati et al. (2016) Cloning and expression of human bone morphogenetic protein-2 gene in Leishmania tarentolae, Biocatalysis and Agricultural Biotechnology https://doi.org/10.1016/j.bcab.2016.01.006.Volume 5, 2016, Pages 199-203, ISSN 1878-8181

Mohajeri, A., Pilehvar-Soltanahmadi, Y., Abdolalizadeh, J., Karimi, P., Zarghami, N. (2016). Effect of Culture Condition Variables on Human Endostatin Gene Expression in Escherichia coli Using Response Surface Methodology, Jundishapur Journal of Microbiology, Vol. 9, No. 8 (2016).

Nikoomanesh, F., et al (2016) Investigation of bcrI Gene Expression in Candida albicans Isolates by TR-PCR Technique and its Impact on Biofilm Formation JInfection Epidemiology and Medicine2(1), p. 22-24.

Pourhassanali, N., Roshan-Milani, S., Kheradmand, F., Motazakker, M., Bagheri, M.,Saboory, E. (2016) ) Zinc attenuates ethanol-induced Sertoli cell toxicity and apoptosis through caspase-3 mediated pathways. Reproductive Toxicology. 61. Pp97-103.

Tafsiri, E., Darbouy, M., Shadmehr, M..B., Cho, W.C., Karimipoor, M. (2016). Abberent expression of oncogenic and tumor-suppressive microRNAs and their target genes in human adenocarcinoma alveolar basal epithelial cells, Journal of Cancer Research and Therapeutics, Vol. 12, No. 1, 395-400.

Abadi, Z.S.N., Fatemi, F., Khosravani, M., Rahmati, M., Sadroddiny, E (2015). Molecular Cloning of Bone Morphogenetic Protein-2 (BMP- 2) in pGEM-b1 Vector and Transformation into E.coli Rosetta Strain, International Journal of Health Sciences, Vol. 1, No. 3 (2015).

Abouhamzeh, B., et al. (2015) DNA Methylation and Histone Acetylation Patterns in Cultured Bovine Adipose Tissue-Derived Stem Cells (BADSCs). CELL Journal16(4), p. 466-475

Ahmadi, S., Karami, Z., Mohammadian, A., Khosrobakhsh, F., Rostamzadeh, J. (2015) Cholestasis Induced Antinociception and Decreased Gene Expression Of MOR1 in Rat brain. Neuroscience. 284. Pp.78-86.

Karimi, S., et al. (2015) Expression of Two Basic mRNA Biomarkers in Peripheral Blood of Patients with Non-Small Cell Lung Cancer Detected by Real-Time RT-PCR, Individually and Simultaneously Iranian Biomedical Journal. 19(1), p. 17-22.

Khodadad, N., et al. (2015) Prevalence of Influenza A(H1N1)pdm09 Virus Resistant to Oseltamivir in Shiraz, Iran, During 2012-2013 Jundishapur Journal of Microbiology8(8).

Madadgar,O., Nazaktabar, A., Keivanfar, H., Salehi, T.Z., Zadeh, S.L. (2015) Genotyping and determining the distribution of prevalent G and P types of group A bovine rotaviruses between 2010 and 2012 in Iran. 179(3-4) pp.190-196

Moghadam, F.H., et al. (2015) Differentiation of Rat Bone Marrow Mesenchymal Stem Cells into Adipocytes and Cardiomyocytes after Treatment with Platelet Lysate International Journal of Hematology-Oncology and Stem Cell Research.10(1), p. 21-29.

Namroodi, S., Rostami, A., Majidzadeh-Ardebili, K., Langroudi, A.G., Morovvati, A. (2015). Detection of Arctic and European cluster of canine distemper virus in north and center of Iran, Veterinary Research Forum, Vol. 6, No. 3, 199-204 (2015).

Tahoori, M.T., Pourfathollah, A.A., Soleimani, M., Vasheghani-Farahani, E., Mohammadzadeh, A., Amari, A., Hashemi, S.M., mossahebi-mohammadi, M. (2015) Fibroblasts feeder niche and Flt3 Ligand as a novel inducer of plasmacytoid dendritic cells development in vitro. International Immunopharmacology. 24. Pp.474-480.

Mostafavi, H., Khaksarian, Joghataei, M.T., Yoosefee, Soleimannejad, Gholamzaddeh, R., Bahnamiri, S.S.,Hadjighassem cAMP-Epac Pathway Stimulation Modulate Connexin-43 and MicroRNA-21 Expression in Glioma Cells, Basic and Clinical NeuroscienceVol. 6, No. 1 (2015).

Heidary, M., Rakhshi, N., Kakhki, M.P., Behmanesh, M., Sanati, M.H., Sanadgol, N., Kamaladinin, H., Nikravesh, A. (2014) The analysis of correlation between IL-1B gene expression and genotyping in multiple sclerosis patients.

Jahantigh, M., Mazaheri, M., Moghadam, A.Y., Motalleb, G., Najafi, S., Najafi, S., Pourrahmat, E., Rashki, A., Sabagh, K., Sanadgol, N., Talee, R. (2014). Epidermal growth factor receptor gene expression evaluation in colorectal cancer patients, Indian Journal of Cancer, Vol. 51, No. 3, 358-362 (2014).

Mostafavi, H., et al. (2014) Fluoxetin Upregulates Connexin 43 Expression in Astrocyte Basic and Clinical Neuroscience. 5(1), p. 74-79.

Najafi, H., Madadgar, O., Jamshidi, S., Langeroudi, A.G., Lemreski, M.D. (2014). Molecular and clinical study on prevalence of feline herpesvirus type 1 and calicivirus in correlation with feline leukemia and immunodeficiency viruses Veterinary Research Forum, Vol. 5 No. 4, 255-261.

Namroodi, S., Rostami, A., Majidzadeh, A.K., Ghalyanchi, L.A. (2014). A phylogenetic study on the NP gene of detected canine distemper virus in (2008-2011) Iran , Iranian Journal of Veterinary Medicine, Vol. 8, No. 3, 163-168 (2014).